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Direct PCR Technology
PCR w/o DNA Purification?
What is the Direct PCR?
Direct PCR technology facilitates PCR amplification directly from small amount of samples without DNA extraction and purification. It eliminates any sample pretreatment steps, so it is very useful to save time and cost, protect samples from cross contamination or loss when dealing with multiple tissue or samples by eliminating tedious genomic DNA preparation. It allows direct PCR amplification from a variety of samples including whole blood, blood collected on card, saliva, bacteria, mouse tail, tissue, cultured cells or plant etc., by neutralizing DNA polymerase inhibitors present in the samples.

KOMA provides many different type of Direct PCR products. EzWay™ Direct PCR Buffer is compatible with most commercially available any DNA polymerases including Taq, Pfu, Pwo, Tth and even with any type of Hot Start Taq DNA polymerase, so you can use it together with your Taq systems that are already set up in your lab. Also, this Direct PCR Buffer is provided as a type of Direct PCR MasterMix which is already mixed with KOMA Taq enzymes . Just adding your primers and template to this MasterMix, you can go directly to PCR amplification.
Advantages of KOMA Direct PCR
Features EzWay Direct PCR Method Conventional PCR Method
No DNA extraction required ■ □
Save Time and Cost ■ □
Less Risk of DNA Contamination ■ □
No Loss of Samples ■ □
Compatibility with any other Taq Enzymes ■ □
KOMA Direct PCR Applications
  • Viral infection / Molecular diagnostic test
  • Forensic DNA Analysis
  • Single cell diagnostic
  • Blood banking
  • Identity testing
  • Multiplex PCR / SNP detection / PCR-RFLP
  • Sequencing / Cloning
  • Laboratory Automatic PCR
Format BUFFER
Product Direct PCR Buffer Direct PCR Buffer Set
Cat. No K0568001 K0568002
Image Direct PCR Buffer Direct PCR Buffer Set
Features This Direct PCR buffer is substituted for the 10X PCR buffer for Direct PCR. Direct PCR buffer is provided with lysis buffers for treatment of hard tissue samples.
Component Direct PCR Buffer Direct PCR Buffer
Direct Lysis Buffer I
Direct Lysis Buffer II
Magic Buffer
Sample Soft materials :
Blood (whole blood, dried blood, blood on paper), Buccal swab, Saliva, Sputum, Urine,... 		Hard materials :
Mouse tail, Animal tissue, Plant, Cultured cell, Hair root, sperm,....
How to use
  1. Prepare blood.
  2. Use 1ul for PCR
  1. Prepare sample.
  2. Lysis
  3. Use 1ul for PCR
No. of use 125 PCR rx (20ul each)
50 PCR rx (50ul each) 		125 PCR rx (20ul each)
50 PCR rx (50ul each)
Format MASTERMIX
Product Direct MasterMix Direct Hot Taq PCR MasterMix Direct Hot Universal qMasterMix
Cat. No K0568010 K0568210 K0569020
Image Direct MasterMix Direct Hot Taq PCR MasterMix Direct Hot Universal qMasterMix
Features Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. Just add the template and primer to this MasterMix. Direct PCR buffer is premixed with Hot Taq, dNTP, MgCl2 and dye. Just add the template and primer to this MasterMix. It is useful for high specificity. Direct PCR buffer is premixed with Hot Taq, dNTP and MgCl2. Just add the template and primer to this MasterMix. It is for real time PCR without DNA extraction.
Component Direct Taq PCR Master Mix
Magic Buffer 		Direct Hot Taq PCR Master Mix
Magic Buffer 		Direct Hot Universal qMasterMix
Magic Buffer
Sample Most of sample type
(Lysis buffer should be purchased separately.) 		Most of sample type
(Lysis buffer should be purchased separately.) 		Most of sample type
(Lysis buffer should be purchased separately.)
How to use
  1. Prepare sample.
  2. Lysis
  3. Use 1ul for PCR
  1. Prepare sample.
  2. Lysis
  3. Use 1ul for PCR
  1. Prepare sample.
  2. Lysis
  3. Use 1ul for PCR
No. of use 100 PCR rx (10ul each)
40 PCR rx (25ul each) 		100 PCR rx (10ul each)
40 PCR rx (25ul each) 		100 PCR rx (10ul each)
40 PCR rx (25ul each)
Format KIT
Product EzWay™ Mouse Tail Direct PCR Kit EzWay™ Plant Direct PCR Kit
Cat. No K0568600 K0568700
Image EzWay™ Mouse Tail Direct PCR Kit EzWay™ Plant Direct PCR Kit
Features Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. It is optimized for genotyping of transgenic mouse using tail or ear tissue without DNA extraction. Direct PCR buffer is premixed with Taq, dNTP, MgCl2 and dye. It is optimized for plant PCR without DNA extraction.
Component Direct Taq PCR Master Mix
Mouse Tail Lysis buffer
Magic Buffer 		Direct Taq PCR Master Mix
Plant Lysis buffer
Magic Buffer
Sample Mouse tail, ear Plant leaves
How to use
  1. Cut tail.
  2. Lysis for 10 min at 60°C
  3. Vortex and spin down.
  4. Mix 1ul supernatant to the MasterMix.
  1. Cut leaf.
  2. Lysis for 10 min at 85°C
  3. Vortex and spin down.
  4. Mix 1ul supernatant to the MasterMix.
No. of use 100 PCR rx (10ul each)
40 PCR rx (25ul each) 		100 PCR rx (10ul each)
40 PCR rx (25ul each)
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