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EzWay Annexin V-FITC Apoptosis Detection Kit
EzWay Annexin V-FITC Apoptosis Detection Kit
Catalog NumberK29100
PriceLogin
Size100T
TypeKit
Manual/Data Sheet
* This manual on-line is just for reference. The contents of this datasheet are subject to change from lot to lot.
Features
  • Utilizes FITC-conjugated Annexin V protein for detection of cells undergoing apoptosis
  • Annexin V FITC binds to the membranes of apoptotic cells
  • Detection by flow cytometry or fluorescence microscopy
Reading of Result
  • Non-apoptotic cells: Annexin-V negative and PI negative
  • Non-apoptotic cells: Annexin-V negative and PI negative
  • Necrotic cells or late apoptotic cells: Annexin-V positive and PI positive
Kit Contents
  • Annexin V-FITC
  • 5X Binding buffer
  • 5X Binding buffer
How to Use
  1. Cell preparation and rinsing cells with Binding Buffer
  2. Add Annexin V-FITC
  3. Add Propidium Iodide
  4. Incubate at 4℃ in the dark.
  5. Detection
Staining of healthy and apoptotic Jurkat cells using flow cytometry
Jurkat cells were incubated with 0.5ug/ml actinomycin D for 20 hours. Untreated cells are shown in the left panel, apoptotic induced cells are shown in the right panel.
Citation List
  • A MAP kinase pathway is implicated in the pseudohyphal induction by hydrogen peroxide in Candica albicans K Srinivasa, J Kim, S Yee, W Kim, WJ Choi Molecules and Cells, 2012 (abstract)
  • Macrophage-stimulating protein attenuates gentamicin-induced inflammation and apoptosis in human renal proximal tubular epithelial cells KE Lee, EY Kim, CS Kim, JS Choi, EH Bae, SK Ma Biochemical and Biophysical Research Communications 2013 (abstract)
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