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EzWay Transfection Reagent
EzWay Transfection Reagent
Catalog NumberK21000
PriceLogin
Size0.5ml
TypeBuffer & Reagent
Manual/Data Sheet
* This manual on-line is just for reference. The contents of this datasheet are subject to change from lot to lot.
Specification
EzWay™ Transfection Reagent, consisting of a unique cationic lipid has appropriate DNA-binding affinity for reliable gene transfection that is applicable to both preclinical and nonclinical studies. Preparing the liposome-DNA complex (lipoplex) is very easy and rapid.
Also, EzWay™ Transfection Reagent shows relatively stronger DNA-binding affinity and better transfection efficiency than other liposomal reagents.
Sufficient reagent for 250 transfection in a 24-well plate is provided.
  • Cationic lipid liposome-based reagent
  • Low cellular toxicity
  • Efficient siRNA and plasmid DNA delivery
  • Transfection of a wide variety of cell types
  • High transfection efficiency of stable and transient transfections
  • Effective in both serum-containing medium and serum-free medium
  • Ready-to-use reagent
  • Simple protocol
  • Universal use for both in vivo and in vitro
Protocol
Transfection Procedure with EzWay™ Transfection Reagent(24-well)
EzWay Transfection Reagent Protocol
Applications
List of cell lines successfully transfected with EzWayTM Transfection Reagent
Cell Line Cell Type
HeLa Human cervix adenocarcinoma
293A Human kidney cancer
B16BL6 Mouse skin melanoma
B16F10 Mouse skin melanoma
MCF-7 Human breast adenocarcinoma
HepG2 Human liver/hepatocellular carcinoma
Hep-3B Human hepatocellular carcinoma
Chang Human normal liver cell
BT-20 Human breast carcinoma
TC-1 Mouse lung tumor
CT-26 Mouse colon carcinoma
EMT-6 Mouse breast cancer
SK-OV-3 Human ovarian adenocarcinoma
A549 Human lung epithelial carcinoma
MDA-MB-231 Human breast adenocarcinoma
NCI Human lung cancer (carcinoma)
SK-Br-3 Human breast carcinoma
HT-29 Human colorectal adenocarcinoma
Jurkat Human T-cell leukemia
Efficient delivery of DNA to a wide variety of cell lines
Efficient delivery of DNA to a wide variety of cell lines (A)
HeLa B16BL6 (B)
Using the EzWay™ Transfection Reagent or competitor reagent L, cells were transfected with the pDNA, which expresses luciferase (A) and GFP (B) respectively. One microgram of pDNA complexed to EzWay ™(1:4.9 wt ratios of pDNA and lipid) was added to a various cell lines, The cells were transfected for 4 hours and incubated for additional 24 hours. The luciferase activity (A) of the cell lysate was measured with a luminometer and a luciferase assay kit and the GFP expression (B) was determined by fluorescence microscopy.
Knockdown efficacy of EzWay™ Transfection Reagent
Knockdown efficacy of EzWay™ Transfection Reagent
siRNA targeting luciferase was transfected by EzWay™ Transfection Reagent and competitor reagent L into Hep-3B cells. For B16BL6 cells, siRNA targeting luciferase was co-transfected with luciferase gene by two products, respectively. Luciferase-silencing efficiency was calculated relative to untreated cells. (A) shows the luciferase expression, while (B) shows the reduction in luciferase activity. EzWay™ Transfection Reagent delivered significant gene silencing from 10 nM of renilla luciferase siRNA.
Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWay™ Transfection Reagent
Inhibition of GFP expression in cells transfected with GFP targeting siRNA using EzWay™ Transfection Reagent
MDA-MB-231 cells constitutively expressing the GFP gene were transfected with 10 nM final siRNA concentration of GFP-targeting siRNA.
Citation List
  • The expression of damage-regulated autophagy modulator 2 (DRAM2) contributes to autophagy induction JH Yoon, S Her, M Kim, IS Jang, JS Park Molecular Biology Reports (abstract)
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