EzWay™ Gel Extraction Kit is designed for rapid purification of DNA from standard agarose gels. It provides a fast and simple method using advanced silica-based membrane technology for rapid and efficient purification of 100bp-10kb DNA fragments. DNA adsorbs to the silica-membrane in the presence of high salt while contaminants pass through the column. Impurities are efficiently washed away, and the pure DNA is eluted with Elution Buffer or water. The purified DNA is suitable for a variety of applications such as fluorescent DNA sequencing, transformation, restriction mapping, cloning, and labeling.

After addition of Extraction Buffer to agarose gel slice sample and heating at 50-55°C to melt gel, DNA is bound to a silica gel membrane in the presence of high concentration of chaotropic salt. The purified DNA product on the membrane is eluted in elution buffer.