EzWay Plant Direct PCR Kit

PCR Reagents > Direct PCR > Direct PCR Kit

Catalog Number	K0568700
Price	Login
Size	1set
Type	PCR Kit
Manual/Data Sheet

* This manual on-line is just for reference. The contents of this datasheet are subject to change from lot to lot.

Description

EzWay™ Plant Direct PCR Kit enables DNA amplification directly from plant leaves without DNA extraction. Punch to isolate disks of leaf tissue and incubate it in the lysis buffer provided in the kit for 10 minutes and then use 1ul of lysate for PCR. Or just leaf itself (when using 0.5 mm punched leaf disc) can be used as a template directly without lysis step. Direct Taq PCR MasterMix in this kit is a convenient mixture of Taq enzyme, dNTP, MgCl₂, dye, additives and Direct PCR Buffer, so you can just add the leaf extract and primers, and then go for PCR. This kit will save your enormous amounts of material, cost and time from conventional PCR methods using mechanical disruption, organic extraction, column DNA purification, or alcohol precipitation. Also, it reduces risk of handling errors and contamination.

Features

Amplification of genomic DNA from plant leaves without DNA extraction
Just 10 minutes incubation required
No need to add Proteinase K
Simple and fast PCR procedure requiring minimal handling
Saving enormous amounts of material and time
Optimized MasterMix type containing EzWay™ Taq PCR enzyme, dNTP, Direct PCR Buffer, MgCl₂, Red dye and additives
Direct loading of PCR products without adding red dye

Kit Contents

2X EzWay™ Direct Taq PCR MasterMix	1 ml
2.5X Plant Direct Lysis Buffer	1.5 ml
4X Magic Buffer	1 ml

How to use

Punch to isolate disks of leaf tissue and add lysis buffer.
Incubate at 85°C for 10 minutes
Vortex and spin down briefly.
Use 1ul supernatant of lysate and go to PCR directly.

Direct PCR from Arabidopsis leaf:

PCR was performed with 1 ul Arabidopsis leaf lysate using the 1X Plant Lysis Buffer in 20 ul PCR. And, pieces of Arabidopsis leaf with 2 mm Harris Punch were placed directly into 20 ul PCR.
Lane 1, 6 and 11: negative control
Lane 2, 7 and 12: 1uL lysate
Lane 3, 8 and 13: one piece
Lane 4, 9 and 14: two pieces
Lane 5, 10 and 15: three pieces

Direct PCR from Plant leaves:

PCR was performed with 1 ul Nicotiana and Capsisum leaf lysate using the 1X Plant Lysis Buffer in 20 ul PCR. And, pieces of Nicotiana and Capsisum leaf with 2 mm Harris Punch were placed directly into 20 ul PCR.
Lane 1 and 5: 1uL lysate
Lane 2 and 6: one piece
Lane 3 and 7: two pieces
Lane 4 and 8: three pieces