EzWay TMB Substrate Kit (TMB+Substrate Solution)

* This manual on-line is just for reference. The contents of this datasheet are subject to change from lot to lot.

Specification

Most sensitive chromogenic substrate for peroxidase detection.
2 component system which develops a deep blue product when reacted with horseradish peroxidase (HRP) in ELISA.
TMB substrates contains 3,3', 5,5' - Tetramethylbenzidine.

Protocol

Dispense 150ul Color Reaction Mixture into each well (Or apply 50ul TMB solution and 100ul Substrate Solution to each well. Mix well by vortexing or shaking slightly).
After sufficient color development (5-10minutes at room temperature or at 37℃), add 50ul Stop Solution (2M H2SO4) to each well.
Read plates in a microwell plate reader at wavelength setting of 450nm.

Kit Contents

Items	Quantity
TMB Solution in organic base	60ml
Substrate Solution (H2O2 in an acidic buffer)	125ml

Citation List

Upper Respiratory Tract Infection Symptoms in Ultramarathon Runners Not Related to Immunoglobulin Status Peters, Edith M PhD, MSc(Med); Shaik, Junaid MMedSci, MTech, Chiro; Kleinveldt, Neil BSc(Med) Hons Clinical Journal of Sport Medicine: January 2010 - Volume 20 - Issue 1 - pp 39-46 (abstract)
Plasma-activated carbon nanotube-based high sensitivity immunosensors for monitoring Legionella pneumophila by direct detection of maltose binding protein peptidoglycan-associated lipoprotein (MBP-PAL) JY Lee, JH Jin, JH Kim, MJ Kim, CJ Lee, NK Min Biotechnology and Bioengineering (abstract)
Suppression of Toll‐like receptor 4 activation by caffeic acid phenethyl ester is mediated by interference of LPS binding to MD2 SY Kim, JE Koo, YJ Seo, N Tyagi, E Jeong, J Choi British Journal of Pharmacology, 2012 (abstract)
Sulforaphane inhibits the engagement of LPS with TLR4/MD2 complex by preferential binding to Cys133 in MD2 JE Koo, ZY Park, ND Kim, JY Lee Biochemical and Biophysical Research Communications 2013 (abstract)